Running summarizing tools on a new article

Abstract: This protocol describes how to perform colorimetric LAMP / RT-LAMP reactions with homemade buffers, together with home-brewed BstLF and MMLV enzymes. It is based on previous protocols of our lab (see LAMP/RT-LAMP Reaction protocol), but since two of the most common indicators used in colorimetric LAMP (phenol red and neutral red) require weakly buffered reactions, the buffer composition needed to be changed. This was done according to indications by Poole et al (2017). The colorimetric methods rely on the inherent proton production by DNA polymerases during the amplification process, which leads to a drop in the reaction pH that can be followed using these pH indicators. The results can be seen directly with the "naked eye", without the need for equipment or hands-on processing (Tanner et al., 2015).